immortalized human ovarian surface epithelial cell line hose Search Results


immortalized human ovarian surface epithelial cell line hose #t1074  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc immortalized human ovarian surface epithelial cell line hose #t1074
MEG3 modulation affected proliferation and clonogenic capability of high-grade serous ovarian cancer (HGSOC) cells. ( A ) Relative MEG3 expression and ( B ) relative MEG3 subcellular localization assessed by RT-qPCR analysis in a panel of HGSOC cell lines as well as in HOSE (Human ovarian surface <t>epithelial</t> cells) and FT194 (Fallopian Tube epithelial cells). Data from total RNA are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using FT194 as a reference sample. Relative MEG3 expression in nucleus vs. cytoplasm is expressed as Log2FC values calculated with the ΔCt method and compared to FT194 cells; positive values represent nuclear enrichment. ( C ) Relative MEG3 expression assessed by RT-qPCR analysis in HEY and PEO1 cells (HGSOC cell lines) transiently transfected with pMEG3 (MEG3 expression plasmid) and with pcDNA (empty vector). Data are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using pcDNA as reference sample. ( D ) Bar chart representing proliferation assay for HEY-pMEG3 and PEO1-pMEG3 cells compared to respective control cells. Viable cells were counted at 24, 48, and 72 h from transfection. The mean cell proliferation at time x (Tx) was expressed as average percentage increase relative to T = 0 h (T0). ( E ) Clonogenic assay. Bar charts represent differences of clonogenic capability in MEG3 overexpressing cells with respect to control cells and representative pictures of clonogenic assays. For all experiments, bars and error bars refer to mean and SEM (standard error of the mean) of three experiments. To establish statistically significant differences, unpaired t -test was carried out: * p < 0.05; ** p < 0.01.
Immortalized Human Ovarian Surface Epithelial Cell Line Hose #T1074, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immortalized human ovarian surface epithelial cell line hose #t1074/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
immortalized human ovarian surface epithelial cell line hose #t1074 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


MEG3 modulation affected proliferation and clonogenic capability of high-grade serous ovarian cancer (HGSOC) cells. ( A ) Relative MEG3 expression and ( B ) relative MEG3 subcellular localization assessed by RT-qPCR analysis in a panel of HGSOC cell lines as well as in HOSE (Human ovarian surface epithelial cells) and FT194 (Fallopian Tube epithelial cells). Data from total RNA are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using FT194 as a reference sample. Relative MEG3 expression in nucleus vs. cytoplasm is expressed as Log2FC values calculated with the ΔCt method and compared to FT194 cells; positive values represent nuclear enrichment. ( C ) Relative MEG3 expression assessed by RT-qPCR analysis in HEY and PEO1 cells (HGSOC cell lines) transiently transfected with pMEG3 (MEG3 expression plasmid) and with pcDNA (empty vector). Data are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using pcDNA as reference sample. ( D ) Bar chart representing proliferation assay for HEY-pMEG3 and PEO1-pMEG3 cells compared to respective control cells. Viable cells were counted at 24, 48, and 72 h from transfection. The mean cell proliferation at time x (Tx) was expressed as average percentage increase relative to T = 0 h (T0). ( E ) Clonogenic assay. Bar charts represent differences of clonogenic capability in MEG3 overexpressing cells with respect to control cells and representative pictures of clonogenic assays. For all experiments, bars and error bars refer to mean and SEM (standard error of the mean) of three experiments. To establish statistically significant differences, unpaired t -test was carried out: * p < 0.05; ** p < 0.01.

Journal: Cancers

Article Title: Clinical Value of lncRNA MEG3 in High-Grade Serous Ovarian Cancer

doi: 10.3390/cancers12040966

Figure Lengend Snippet: MEG3 modulation affected proliferation and clonogenic capability of high-grade serous ovarian cancer (HGSOC) cells. ( A ) Relative MEG3 expression and ( B ) relative MEG3 subcellular localization assessed by RT-qPCR analysis in a panel of HGSOC cell lines as well as in HOSE (Human ovarian surface epithelial cells) and FT194 (Fallopian Tube epithelial cells). Data from total RNA are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using FT194 as a reference sample. Relative MEG3 expression in nucleus vs. cytoplasm is expressed as Log2FC values calculated with the ΔCt method and compared to FT194 cells; positive values represent nuclear enrichment. ( C ) Relative MEG3 expression assessed by RT-qPCR analysis in HEY and PEO1 cells (HGSOC cell lines) transiently transfected with pMEG3 (MEG3 expression plasmid) and with pcDNA (empty vector). Data are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using pcDNA as reference sample. ( D ) Bar chart representing proliferation assay for HEY-pMEG3 and PEO1-pMEG3 cells compared to respective control cells. Viable cells were counted at 24, 48, and 72 h from transfection. The mean cell proliferation at time x (Tx) was expressed as average percentage increase relative to T = 0 h (T0). ( E ) Clonogenic assay. Bar charts represent differences of clonogenic capability in MEG3 overexpressing cells with respect to control cells and representative pictures of clonogenic assays. For all experiments, bars and error bars refer to mean and SEM (standard error of the mean) of three experiments. To establish statistically significant differences, unpaired t -test was carried out: * p < 0.05; ** p < 0.01.

Article Snippet: The immortalized human ovarian surface epithelial cell line (HOSE, #T1074) was purchased from Applied Biological Materials Inc. (ABM, Richmond, BC, Canada), whereas the immortalized Fallopian tube secretory epithelial cell line FT194 was kindly provided by Dr. MS Zannini, with the authorization of Dr. R Drapkin (Boston, MA, USA).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Plasmid Preparation, Proliferation Assay, Control, Clonogenic Assay